Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter
Por um escritor misterioso
Last updated 29 junho 2024
![Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter](https://www.jneurosci.org/content/jneuro/23/18/7143/F3.large.jpg)
The melanocortin-4 receptor (MC4-R) is an important regulator of energy homeostasis, and evidence suggests that MC4-R-expressing neurons are downstream targets of leptin action. MC4-Rs are broadly expressed in the CNS, and the distribution of MC4-R mRNA has been analyzed most extensively in the rat. However, relatively little is known concerning chemical profiles of MC4-R-expressing neurons. The extent to which central melanocortins act presynaptically or postsynaptically on MC4-Rs is also unknown. To address these issues, we have generated a transgenic mouse line expressing green fluorescent protein (GFP) under the control of the MC4-R promoter, using a modified bacterial artificial chromosome. We have confirmed that the CNS distribution of GFP-producing cells is identical to that of MC4-R mRNA in wild-type mice and that nearly all GFP-producing cells coexpress MC4-R mRNA. For example, cells coexpressing GFP and MC4-R mRNA were distributed in the paraventricular hypothalamic nucleus (PVH) and the dorsal motor nucleus of the vagus (DMV). MC4-R promotor-driven GFP expression was found in PVH cells producing thyrotropin-releasing hormone and in cholinergic DMV cells. Finally, we have observed that a synthetic MC3/4-R agonist, MT-II, depolarizes some GFP-expressing cells, suggesting that MC4-Rs function postsynaptically in some instances and may function presynaptically in others. These studies extend our knowledge of the distribution and function of the MC4-R. The transgenic mouse line should be useful for future studies on the role of melanocortin signaling in regulating feeding behavior and autonomic homeostasis.
![Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter](https://www.jneurosci.org/content/jneuro/23/18/7143/F7.large.jpg)
Transgenic Mice Expressing Green Fluorescent Protein under the
![Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter](https://www.cell.com/cms/attachment/7fed8fd2-3146-4574-adb8-841eb6afdc06/gr7.jpg)
Divergence of Melanocortin Pathways in the Control of Food Intake
![Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter](https://www.jneurosci.org/content/jneuro/33/2/464/F2.large.jpg)
Melanocortin-4 Receptor Regulates Hippocampal Synaptic Plasticity
![Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter](https://media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41598-021-83932-4/MediaObjects/41598_2021_83932_Fig3_HTML.png)
Melanocortin receptor activation alleviates amyloid pathology and
![Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter](https://www.researchgate.net/publication/6915927/figure/fig2/AS:339817675149326@1458030255458/Reduction-of-green-fluorescent-protein-GFP-expression-and-increase-in-production-of.png)
Reduction of green fluorescent protein (GFP) expression and
![Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter](https://www.jneurosci.org/content/jneuro/23/18/7143/F2.large.jpg)
Transgenic Mice Expressing Green Fluorescent Protein under the
![Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter](https://www.cell.com/cms/attachment/586edf1f-9ae1-43d8-803d-9d81bb1a1396/fx1_lrg.jpg)
Melanocortin 4 Receptors Reciprocally Regulate Sympathetic and
![Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter](https://media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41592-023-01896-x/MediaObjects/41592_2023_1896_Fig2_HTML.png)
Hardwiring tissue-specific AAV transduction in mice through
![Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter](https://onlinelibrary.wiley.com/cms/asset/2618a673-0897-41c6-ab8a-6303d0225d45/mfig006.jpg)
Journal of Comparative Neurology Systems Neuroscience Journal
![Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter](https://www.cell.com/cms/attachment/69428294-ca55-48a5-940a-c66bf3b58ef7/gr1.jpg)
Molecular Therapy of Melanocortin-4-Receptor Obesity by an
![Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter](https://ars.els-cdn.com/content/image/1-s2.0-S0021925820318044-gr10.jpg)
The stimulatory G protein Gsα is required in melanocortin 4
![Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter](https://www.cell.com/cms/attachment/94094f78-7d4e-47c1-8960-eada12ff8f84/gr2.jpg)
Divergence of Melanocortin Pathways in the Control of Food Intake
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