Terraria: How To Summon And Defeat Brain Of Cthulhu

Here's how Terraria players can summon and defeat Brain of Cthulhu.

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Terraria 1.4.4.9 How To Summon Brain of Cthulhu (2 WAYS)

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How to summon and defeat the Eye of Cthulhu in Terraria

I can't summon Brain of Cthulhu. I once used the Bloody Spine died and I can't do it anymore. Here is my arena for it. Can I use the Bloody Spine once