Metabolic engineering of Deinococcus radiodurans for pinene
Por um escritor misterioso
Last updated 01 julho 2024
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Background The objective of this work was to engineer Deinococcus radiodurans R1 as a microbial cell factory for the production of pinene, a monoterpene molecule prominently used for the production of fragrances, pharmaceutical products, and jet engine biofuels. Our objective was to produce pinene from glycerol, an abundant by-product of various industries. Results To enable pinene production in D. radiodurans, we expressed the pinene synthase from Abies grandis, the geranyl pyrophosphate (GPP) synthase from Escherichia coli, and overexpressed the native 1-deoxy-d-xylulose 5-phosphate synthase. Further, we disrupted the deinoxanthin pathway competing for the substrate GPP by either inactivating the gene dr0862, encoding phytoene synthase, or substituting the native GPP synthase with that of E. coli. These manipulations resulted in a D. radiodurans strain capable of producing 3.2 ± 0.2 mg/L pinene in a minimal medium supplemented with glycerol, with a yield of 0.13 ± 0.04 mg/g glycerol in shake flask cultures. Additionally, our results indicated a higher tolerance of D. radiodurans towards pinene as compared to E. coli. Conclusions In this study, we successfully engineered the extremophile bacterium D. radiodurans to produce pinene. This is the first study demonstrating the use of D. radiodurans as a cell factory for the production of terpenoid molecules. Besides, its high resistance to pinene makes D. radiodurans a suitable host for further engineering efforts to increase pinene titer as well as a candidate for the production of the other terpenoid molecules.
![Metabolic engineering of Deinococcus radiodurans for pinene](https://orbit.dtu.dk/files-asset/340122403/16558_2b9a7411.jpg/)
Carsten Jers — Welcome to DTU Research Database
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PDF] Metabolic Engineering of Deinococcus radiodurans for the Production of Phytoene.
![Metabolic engineering of Deinococcus radiodurans for pinene](https://www.frontiersin.org/files/Articles/659233/fmicb-12-659233-HTML/image_m/fmicb-12-659233-g001.jpg)
Frontiers Lack of the Bacterial Phytochrome Protein Decreases Deinococcus radiodurans Resistance to Mitomycin C
![Metabolic engineering of Deinococcus radiodurans for pinene](https://journals.asm.org/cms/10.1128/aem.00038-22/asset/913ed546-7232-467f-9b55-fb3029fef78d/assets/images/large/aem.00038-22-f001.jpg)
A Novel Small RNA, DsrO, in Deinococcus radiodurans Promotes Methionine Sulfoxide Reductase (msrA) Expression for Oxidative Stress Adaptation
![Metabolic engineering of Deinococcus radiodurans for pinene](https://www.coursehero.com/thumb/55/e4/55e4c6cb64a8b23fe6049e763776cc263c49abf9_180.jpg)
Week 1 Discussion Micro.docx - Microbiology is both a basic and an applied science. It is not a single subject! It has many areas of specialization
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Engineering Robustness of Microbial Cell Factories - Gong - 2017 - Biotechnology Journal - Wiley Online Library
![Metabolic engineering of Deinococcus radiodurans for pinene](https://onlinelibrary.wiley.com/cms/asset/20905222-bc46-46f4-aabf-aa6c4e1bce8f/biot201700014-fig-0002-m.jpg)
Engineering Robustness of Microbial Cell Factories - Gong - 2017 - Biotechnology Journal - Wiley Online Library
![Metabolic engineering of Deinococcus radiodurans for pinene](https://pubs.acs.org/cms/10.1021/sb4001382/asset/images/large/sb-2013-001382_0001.jpeg)
Microbial Synthesis of Pinene
![Metabolic engineering of Deinococcus radiodurans for pinene](https://www.aablocks.com/storage/structure/5078/105078.png)
53-84-9, MFCD00150381, NAD+
![Metabolic engineering of Deinococcus radiodurans for pinene](https://pub.mdpi-res.com/microorganisms/microorganisms-09-00044/article_deploy/html/images/microorganisms-09-00044-g003.png?1609294122)
Microorganisms, Free Full-Text
![Metabolic engineering of Deinococcus radiodurans for pinene](https://www.science.org/cms/10.1126/science.1103185/asset/89bb2677-b1b2-4762-9492-3ec35ecc78ff/assets/graphic/306_1025_f4.jpeg)
Accumulation of Mn(II) in Deinococcus radiodurans Facilitates Gamma-Radiation Resistance
![Metabolic engineering of Deinococcus radiodurans for pinene](https://media.springernature.com/w290h158/springer-static/image/art%3A10.1186%2Fs12934-021-01731-y/MediaObjects/12934_2021_1731_Figa_HTML.png)
Microbial Cell Factories
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Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories, Bioresources and Bioprocessing
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Full article: A review on biofiltration techniques: recent advancements in the removal of volatile organic compounds and heavy metals in the treatment of polluted water
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